詳細(xì)說(shuō)明

Product Description

This product thermostable reverse transcriptase is modified by site directed mutation on the basis of mouse virus reverse transcriptase, removes RNaseH activity, and can withstand high temperature reaction at 65 ° C. Compared with the conventional reverse transcription reaction under low temperature, high temperature reverse transcription can significantly open the secondary structure of RNA, improve the amplification performance of complex RNA template, improve the length and yield of reverse transcription cDNA, and improve the sensitivity of subsequent detection.

Activity Definition

With poly (RA) as template and oligo (DT) as primer, the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into acid insoluble precipitate in 10 minutes at 37 ° C is defined as an active unit.

Please click the link below to download the manual

M602 Thermostable Reverse Transcriptase說(shuō)明書(shū).pdf